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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
03/05/2010 |
Data da última atualização: |
02/08/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
KITAJIMA, E. W.; CALEGARIO, R. F.; FREITAS-ASTUA, J.; ALBERTI, G. |
Afiliação: |
Elliot Watanabe Kitajima, ESALQ; Renata F. Calegario, ESALQ; JULIANA DE FREITAS ASTUA, CNPMF; Gerd Alberti, Uni- Greitswald. |
Título: |
Electron microscopic evidences that Citrus leprosis virus, cytoplasmic type (CiLV-C) only circulates in the vector Brevipalpus phoenicis (Acari: Tenuipalpidae) while other nuclear type Brevipalpus transmitted viruses do replicate. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In:MILBENKUNDLICHES KOLLOQUIUM, 7., 2009, Posen. Abstracts... Posen: Adam Mickiewicz Universität, 2009. p. 28-29. |
Idioma: |
Inglês |
Conteúdo: |
Electron microscopic (EM) examination of Brevipalpus phoenicis (Acari: Tenuipalpidae) colonizing Citrus leprosis virus, cytoplasmic type (CiLV-C)-infected sweet orange plants permitted the detection of bacilliform, virus-like particles only between membrane of adjacent cells in the vicinity of the prosomal gland complex and the midgut. The viral nature of these particles was assessed by immunolocalization using anti-CiLV-C capsid proteins. To explain the viral route from the midgut lumen to the duct of the prosomal gland complex two possibilities were considered: (a) intracellular, in which viral particles could be endo- and exocyted in the epithelium of the midgut and prosomal gland complex. (b) Paracellular, by which virions could pass through epithelial cell junctions in a process resembling diapedesis of leucocytes from the capilary vessels in vertebrates. Virions could be transported from midgut to the prosomal glands by a flux induced by the mite movement. Since no virions were ever found within cells. were favor the paracellar route. Also, because we could not detect the characteristic electron dense and vacuolated viroplasma in the mite tissues, we believe that CiLV-C does not multiply en the vector, just circulating in the mite body. Other biological and molecular evidences are in line with this thought. On the other hand, EM observations on several other Brevipalpus-transmitted viruses (BTV) of the nuclear type (nuclear viroplasm) as Coffee ringspot virus (CoRSV). Clerodendrum chlorotic spot virus (CICSV), citrus leprosis virus-nuclear type (CiLV-N) and Orchid fleck virus (OFV) revealed the presence of the rod-shaped virus-like particles in the nucleus and cytoplasm, and the lypical electron lucent nuclear viroplasm in the cells of the epithelium of the midgut and of the prosomal gland complex, as it occurs in infected leaf cells. The detection of the viroplasm strongly suggests that these viruses multiply in some tissues of the mite vector (B. phoenicis for CoRSV, CICSV, CiLV-N) and B. califomicus, for OFV). MenosElectron microscopic (EM) examination of Brevipalpus phoenicis (Acari: Tenuipalpidae) colonizing Citrus leprosis virus, cytoplasmic type (CiLV-C)-infected sweet orange plants permitted the detection of bacilliform, virus-like particles only between membrane of adjacent cells in the vicinity of the prosomal gland complex and the midgut. The viral nature of these particles was assessed by immunolocalization using anti-CiLV-C capsid proteins. To explain the viral route from the midgut lumen to the duct of the prosomal gland complex two possibilities were considered: (a) intracellular, in which viral particles could be endo- and exocyted in the epithelium of the midgut and prosomal gland complex. (b) Paracellular, by which virions could pass through epithelial cell junctions in a process resembling diapedesis of leucocytes from the capilary vessels in vertebrates. Virions could be transported from midgut to the prosomal glands by a flux induced by the mite movement. Since no virions were ever found within cells. were favor the paracellar route. Also, because we could not detect the characteristic electron dense and vacuolated viroplasma in the mite tissues, we believe that CiLV-C does not multiply en the vector, just circulating in the mite body. Other biological and molecular evidences are in line with this thought. On the other hand, EM observations on several other Brevipalpus-transmitted viruses (BTV) of the nuclear type (nuclear viroplasm) as Coffee ringspot virus (CoRSV). ... Mostrar Tudo |
Thesagro: |
Brevipalpus Phoenicis; Vírus. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/748556/1/Electron-microscopic-evidences-that-Citrus-leprosis-virus-cytoplasmic-type.pdf
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Marc: |
LEADER 02788nam a2200169 a 4500 001 1748556 005 2023-08-02 008 2009 bl uuuu u00u1 u #d 100 1 $aKITAJIMA, E. W. 245 $aElectron microscopic evidences that Citrus leprosis virus, cytoplasmic type (CiLV-C) only circulates in the vector Brevipalpus phoenicis (Acari$bTenuipalpidae) while other nuclear type Brevipalpus transmitted viruses do replicate.$h[electronic resource] 260 $aIn:MILBENKUNDLICHES KOLLOQUIUM, 7., 2009, Posen. Abstracts... Posen: Adam Mickiewicz Universität, 2009. p. 28-29.$c2009 520 $aElectron microscopic (EM) examination of Brevipalpus phoenicis (Acari: Tenuipalpidae) colonizing Citrus leprosis virus, cytoplasmic type (CiLV-C)-infected sweet orange plants permitted the detection of bacilliform, virus-like particles only between membrane of adjacent cells in the vicinity of the prosomal gland complex and the midgut. The viral nature of these particles was assessed by immunolocalization using anti-CiLV-C capsid proteins. To explain the viral route from the midgut lumen to the duct of the prosomal gland complex two possibilities were considered: (a) intracellular, in which viral particles could be endo- and exocyted in the epithelium of the midgut and prosomal gland complex. (b) Paracellular, by which virions could pass through epithelial cell junctions in a process resembling diapedesis of leucocytes from the capilary vessels in vertebrates. Virions could be transported from midgut to the prosomal glands by a flux induced by the mite movement. Since no virions were ever found within cells. were favor the paracellar route. Also, because we could not detect the characteristic electron dense and vacuolated viroplasma in the mite tissues, we believe that CiLV-C does not multiply en the vector, just circulating in the mite body. Other biological and molecular evidences are in line with this thought. On the other hand, EM observations on several other Brevipalpus-transmitted viruses (BTV) of the nuclear type (nuclear viroplasm) as Coffee ringspot virus (CoRSV). Clerodendrum chlorotic spot virus (CICSV), citrus leprosis virus-nuclear type (CiLV-N) and Orchid fleck virus (OFV) revealed the presence of the rod-shaped virus-like particles in the nucleus and cytoplasm, and the lypical electron lucent nuclear viroplasm in the cells of the epithelium of the midgut and of the prosomal gland complex, as it occurs in infected leaf cells. The detection of the viroplasm strongly suggests that these viruses multiply in some tissues of the mite vector (B. phoenicis for CoRSV, CICSV, CiLV-N) and B. califomicus, for OFV). 650 $aBrevipalpus Phoenicis 650 $aVírus 700 1 $aCALEGARIO, R. F. 700 1 $aFREITAS-ASTUA, J. 700 1 $aALBERTI, G.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registros recuperados : 111 | |
3. | | FREITAS-ÁSTUA, J.; NICOLINI, F.; BASTIANEL, M.; KITAJIMA, E. W. Interações entre o vírus da leprose dos citros e seu vetor Brevipalpus phoenicis. Summa Phytopathologica, Botucatu, v. 34, , p. 128, fev. 2008. Suplemento. Edição dos Resumos do XXXI Congresso Paulista de Fitopatologia, Campinas, fev. 2008.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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5. | | KUBO, K. S.; FREITAS-ÁSTUA, J.; KITAGIMA, E. W. Detecção do Clerodendron chlorotic sport virus in planta e no ácaro vetor. Summa Phytopathologica, Botucatu, v. 34, , p. 97, fev. 2008. Suplemento. Edição dos Resumos do XXXI Congresso Paulista de Fitopatologia, Campinas, fev. 2008.
R 301Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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6. | | BASTIANEL, M.; NOVELLI, V. M.; FREITAS-ASTUA, J. de. Field assessments reveal different levels of susceptibility among mandarins and hybrids to Citrus Leprosis Virus c. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 129.PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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8. | | MASCARENHAS, G.; BARBOSA, C. de J.; KITAJIMA, E. W.; FREITAS-ÁSTUA, J. Alta eficiência de transmissão mecânica e por vetor do vírus da pinta verde (PFGSV) para maracujazeiro. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 911.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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9. | | KITAJIMA, E. W.; CHAGAS, C. M.; HARAKAWA, R.; FREITAS-ASTÚA, J.; RODRIGUES, J. C. V. Citrus leprosis in Florida may have been of the nuclear type. Virus Reviews & Research, Rio de Janeiro, v. 14, p. 90, 2009. Suplemento 1. Trabalho apresentado no XX Encontro Nacional de Virologia, Brasília, DF. Resumo 098.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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10. | | TASSI, A. D.; FREITAS-ASTÚA, J.; JADÃO, A.; KITAJIMA, E. W. Citrus leprosis virus (CILV-C) vector relationship assessed by bean as test plant. Virus Reviews & Research, Rio de Janeiro, v. 14, p. 90, 2009. Suplplement 1. Trabalho apresentado no XX Encontro Nacional de Virologia, Brasília, DF. Resumo 097Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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11. | | RODRIGUES, A. S.; BARBOSA, C. de J.; SOARES FILHO, W. dos S.; FREITAS-ASTUA, J. de. Citrus rootstock hybrids tolerants to citrus tristeza virus and Bahia bark scaling disease. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 106_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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15. | | NOVELLI, V. M.; FREITAS-ASTÚA, J.; KITAJIMA, E. W.; GUIDOTTI, D. F. S.; MACHADO, M. A. Transmissão do vírus da leprose dos citros (CiLV-C) por populações apossimbióticas de B. phoenicis. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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16. | | KUBO, K. S.; ARENA, G. D.; KITAJIMA, E. W; MACHADO, M. A.; FREITAS-ASTUA, J. de. Transmission of citrus leprosis virus c (CiLV-C) to Arabidopsis thaliana by Brevipalpus phoenicis mites. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 085 PS1
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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17. | | NOVELLI, V. M.; ALVES, A.; BASSANEZI, R. B.; BASTIANEL, M.; FREITAS-ÁSTUA, J. Validação preliminar de um método sensível para a detecção do vírus da leprose (CiLV-C) em ácaros Brevipalpus phoenicis provenientes de pomates de citros orgânicos e convencionais. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 926.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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20. | | FREITAS-ASTUA, J.; ASTUA-MONGE, G.; POLSTON, J. E.; HIEBERT, E. A simple and reliable method for the screening of transgenic tobacco plants. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 7, p. 893-896, jul. 2003 Notas Científicas.
Título em português: Método simples e confiável para avaliação de plantas de fumo transgênicas.Biblioteca(s): Embrapa Unidades Centrais. |
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Registros recuperados : 111 | |
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